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Last updated: 2016/12/4
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PPP1R13L polyclonal antibody

  • Catalog # : PAB10243
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13L.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 780-797 of human PPP1R13L.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Mouse
  • Specificity:
  • This affinity-purified antibody is directed against the human iASPP protein. Expect reactivity against both isoform 1 and isoform 2.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:40000-1:160000)
    Western Blot (1:1000-1:5000)
    Immunohistochemistry (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PPP1R13L polyclonal antibody (Cat # PAB10243) shows detection of a band at ~100 KDa (arrowhead) corresponding to isoform 1 of PPP1R13L in MCF-7 whole cell lysates.
    Preincubation with immunizing peptide blocks specific band staining (data not shown).
    Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
    After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C.
    The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of PPP1R13L polyclonal antibody (Cat # PAB10243) shows strong cytoplasmic and membranous staining of myocytes in human heart tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_006654;Q8WUF5
  • Gene Name:
  • PPP1R13L
  • Gene Alias:
  • IASPP,NKIP1,RAI
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 13 like
  • Gene Summary:
  • IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM
  • Other Designations:
  • NFkB interacting protein 1,RelA-associated inhibitor,inhibitor of apoptosis stimulating protein of p53,protein phosphatase 1, regulatory subunit 13 like
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