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WRNIP1 polyclonal antibody

  • Catalog # : PAB10242
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of WRNIP1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human WRNIP1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 72.2, 69.5
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Specificity:
  • The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 KDa and 69.5 KDa, respectively.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Fig. 1
    Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) to detect human WRNIP1 present in a HEK293 whole cell lysate.
    Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
    Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 KDa is detected.
    The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.
    See Figure 2 for the results of peptide competition experiments.
    The blot was incubated with a 1 : 200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
  • Western Blot
  • Western Blot
  • Fig. 2
    Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) with and without pre-incubation with blocking peptide.
    Testing was performed on antiserum prior to affinity purification.
    Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WRNIP1.
    Approximately 30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.
    Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 KDa is detected.
    The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q96S55;CAH73664
  • Gene Name:
  • WRNIP1
  • Gene Alias:
  • FLJ22526,RP11-420G6.2,WHIP,bA420G6.2
  • Gene Description:
  • Werner helicase interacting protein 1
  • Gene Summary:
  • Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000015944,OTTHUMP00000015945,Werner helicase interacting protein,putative helicase RUVBL
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