WRNIP1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Fig. 1
Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) to detect human WRNIP1 present in a HEK293 whole cell lysate.
Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 KDa is detected.
The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.
See Figure 2 for the results of peptide competition experiments.
The blot was incubated with a 1 : 200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
Western Blot
Fig. 2
Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) with and without pre-incubation with blocking peptide.
Testing was performed on antiserum prior to affinity purification.
Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WRNIP1.
Approximately 30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 KDa is detected.
The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WRNIP1.
Immunogen
A synthetic peptide corresponding to internal region of human WRNIP1.
Host
Rabbit
Theoretical MW (kDa)
72.2, 69.5
Reactivity
Human, Monkey, Mouse, Rat
Specificity
The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 KDa and 69.5 KDa, respectively.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Fig. 1
Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) to detect human WRNIP1 present in a HEK293 whole cell lysate.
Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 KDa is detected.
The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.
See Figure 2 for the results of peptide competition experiments.
The blot was incubated with a 1 : 200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.Western Blot
Fig. 2
Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) with and without pre-incubation with blocking peptide.
Testing was performed on antiserum prior to affinity purification.
Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WRNIP1.
Approximately 30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 KDa is detected.
The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — WRNIP1
Entrez GeneID
56897Protein Accession#
Q96S55;CAH73664Gene Name
WRNIP1
Gene Alias
FLJ22526, RP11-420G6.2, WHIP, bA420G6.2
Gene Description
Werner helicase interacting protein 1
Omim ID
608196Gene Ontology
HyperlinkGene Summary
Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000015944|OTTHUMP00000015945|Werner helicase interacting protein|putative helicase RUVBL
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Interactome
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Publication Reference
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A novel protein interacts with the Werner's syndrome gene product physically and functionally.
Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T.
The Journal of Biological Chemistry 2001 Jun; 276(23):20364.
Application：IP, WB-Tr, Human , HEK 293 cells.
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A novel protein interacts with the Werner's syndrome gene product physically and functionally.
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