USP18 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using USP18 polyclonal antibody (Cat # PAB10241) shows detection of a band ~43 KDa band (arrowhead) believed to be to USP18 in lysates from HeLa nuclear extracts (Lane 1) and whole cell lysates from HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and HEK293 (Lane 5).
Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 KDa and 50 KDa markers is expected to be USP18.
The other bands are probably non-specific.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP18.
Immunogen
A synthetic peptide corresponding to amino acids 40-49 of human USP18.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human, Mouse, Orangutan
Specificity
This affinity-purified antibody is directed against human UBP43 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using USP18 polyclonal antibody (Cat # PAB10241) shows detection of a band ~43 KDa band (arrowhead) believed to be to USP18 in lysates from HeLa nuclear extracts (Lane 1) and whole cell lysates from HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and HEK293 (Lane 5).
Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 KDa and 50 KDa markers is expected to be USP18.
The other bands are probably non-specific.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP18
Entrez GeneID
11274Protein Accession#
Q9UMW8;NP_059110Gene Name
USP18
Gene Alias
ISG43, UBP43
Gene Description
ubiquitin specific peptidase 18
Omim ID
607057Gene Ontology
HyperlinkGene Summary
USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM
Other Designations
ubiquitin specific protease 18
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Interactome
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Publication Reference
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The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase.
Tokarz S, Berset C, La Rue J, Friedman K, Nakayama K, Nakayama K, Zhang DE, Lanker S.
The Journal of Biological Chemistry 2004 Nov; 279(45):46424.
Application：WB, Mouse, MEFs.
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Human and mouse proteases: a comparative genomic approach.
Puente XS, Sanchez LM, Overall CM, Lopez-Otin C.
Nature Reviews. Genetics 2003 Jul; 4(7):544.
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Cloning and characterization of human ubiquitin-processing protease-43 from terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH.
Kang D, Jiang H, Wu Q, Pestka S, Fisher PB.
Gene 2001 Apr; 267(2):233.
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Cloning and characterization of a novel human ubiquitin-specific protease, a homologue of murine UBP43 (Usp18).
Schwer H, Liu LQ, Zhou L, Little MT, Pan Z, Hetherington CJ, Zhang DE.
Genomics 2000 Apr; 65(1):44.
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RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response.
Li XL, Blackford JA, Judge CS, Liu M, Xiao W, Kalvakolanu DV, Hassel BA.
The Journal of Biological Chemistry 2000 Mar; 275(12):8880.
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The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase.
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