TLK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TLK1.
Immunogen
A synthetic peptide corresponding to amino acids 737-751 of human TLK1.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Orangutan, Rat
Specificity
This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. This antibody will react with all isoforms of Tlk-1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using TLK1 polyclonal antibody (Cat # PAB10238) shows detection of aband ~ 64 KDa corresponding to isoform 3 of human TLK1 (arrowhead).
Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).
Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 250 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TLK1
Entrez GeneID
9874Protein Accession#
Q9UKI8;NP_036422(isoform1)Gene Name
TLK1
Gene Alias
KIAA0137, PKU-beta
Gene Description
tousled-like kinase 1
Omim ID
608438Gene Ontology
HyperlinkGene Summary
The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM
Other Designations
SNARE protein kinase SNAK|serine threonine protein kinase
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Interactome
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Publication Reference
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TLK1B is elevated with eIF4E overexpression in breast cancer.
Norton KS, McClusky D, Sen S, Yu H, Meschonat C, Debenedetti A, Li BD.
The Journal of Surgical Research 2004 Jan; 116(1):98.
Application:WB-Ti, Human, Human breast cancer.
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Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K.
Oncogene 2003 Sep; 22(38):5927.
Application:IP, WB-Tr, Human, AT1ABR, C2ABR, HCT-115, L3 cells.
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Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.
Groth A, Lukas J, Nigg EA, Sillje HH, Wernstedt C, Bartek J, Hansen K.
The EMBO Journal 2003 Apr; 22(7):1676.
Application:IP, WB-Tr, Human, AT fibroblasts, HCT-15, HeLa, HT-29, U-2 OS cells.
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TLK1B is elevated with eIF4E overexpression in breast cancer.
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