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Last updated: 2017/5/21
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TLK1 polyclonal antibody

  • Catalog # : PAB10238
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TLK1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 737-751 of human TLK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Human, Mouse, Orangutan, Rat
  • Specificity:
  • This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. This antibody will react with all isoforms of Tlk-1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using TLK1 polyclonal antibody (Cat # PAB10238) shows detection of aband ~ 64 KDa corresponding to isoform 3 of human TLK1 (arrowhead).
    Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).
    Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 250 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9874
  • Protein Accession#:
  • Q9UKI8;NP_036422(isoform1)
  • Gene Name:
  • TLK1
  • Gene Alias:
  • KIAA0137,PKU-beta
  • Gene Description:
  • tousled-like kinase 1
  • Gene Summary:
  • The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM
  • Other Designations:
  • SNARE protein kinase SNAK,serine threonine protein kinase
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