Western blot using FBXW11 polyclonal antibody (Cat # PAB10237) shows detection of mouse and human FBXW11 (arrowhead) in NIH/3T3 (Lane 1) and 293 (Lane 2) whole cell lysates, respectively. The band appears as a 58 KDa protein, although a 62.1 KDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1 : 200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1 : 20,000 in blocking buffer for 1 h at 4°C.
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. [provided by RefSeq
F-box and WD-40 domain protein 11,F-box and WD-40 domain protein 1B,F-box protein Fbw1b,beta-transducin repeat-containing protein 2