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Last updated: 2017/8/20
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SFRP1 polyclonal antibody

  • Catalog # : PAB10235
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SFRP1.
  • Immunogen:
  • A synthetic peptide corresponding to 12 amino acids region of human SFRP1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human SFRP protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:200-1:2000)
    Immunohistochemistry (1:800)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using SFRP1 polyclonal antibody (Cat # PAB10235) shows detection of a band ~37 KDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells.
    Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of SFRP1 polyclonal antibody overnight at 4°C.
    Signal was detected using standard techniques.
    Personnel communication Becky Mercer, University of Columbia.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of SFRP1 polyclonal antibody (Cat # PAB10235) was used at a 1 : 800 dilution for 20 min to detect SFRP1 in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Goat-anti-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6422
  • Protein Accession#:
  • O14779;NP_003003
  • Gene Name:
  • SFRP1
  • Gene Alias:
  • FRP,FRP-1,FRP1,FrzA,SARP2
  • Gene Description:
  • secreted frizzled-related protein 1
  • Gene Summary:
  • This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq
  • Other Designations:
  • secreted apoptosis-related protein 2
  • Gene Pathway
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