Western blot using SFRP1 polyclonal antibody (Cat # PAB10235) shows detection of a band ~37 KDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of SFRP1 polyclonal antibody overnight at 4°C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
Immunohistochemistry of SFRP1 polyclonal antibody (Cat # PAB10235) was used at a 1 : 800 dilution for 20 min to detect SFRP1 in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Goat-anti-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq