Products

Western Blot (Cell lysate)

Western blot using SFRP1 polyclonal antibody (Cat # PAB10235) shows detection of a band ~37 KDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells.
Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of SFRP1 polyclonal antibody overnight at 4°C.
Signal was detected using standard techniques.
Personnel communication Becky Mercer, University of Columbia.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemistry of SFRP1 polyclonal antibody (Cat # PAB10235) was used at a 1 : 800 dilution for 20 min to detect SFRP1 in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Goat-anti-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

6422

Protein Accession#

O14779;NP_003003

Gene Name

SFRP1

Gene Alias

FRP, FRP-1, FRP1, FrzA, SARP2

Gene Description

secreted frizzled-related protein 1

Omim ID

604156

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq

Other Designations

secreted apoptosis-related protein 2