RBX1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RBX1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 94-108 of human RBX1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — RBX1
Entrez GeneID
9978Protein Accession#
NP_055063;P62877Gene Name
RBX1
Gene Alias
BA554C12.1, MGC13357, MGC1481, RNF75, ROC1
Gene Description
ring-box 1
Omim ID
603814Gene Ontology
HyperlinkGene Summary
This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq
Other Designations
OTTHUMP00000028983|RING box protein 1|RING finger protein|ZYP protein|regulator of cullins 1
-
Interactome
-
Pathway
-
Publication Reference
-
The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.
Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y.
Molecular and Cellular Biology 2000 Nov; 20(21):8185.
Application:IF, Human, U2OS cells.
-
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.
Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ.
Genes & Development 1999 Jun; 13(12):1614.
-
Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.
Skowyra D, Koepp DM, Kamura T, Conrad MN, Conaway RC, Conaway JW, Elledge SJ, Harper JW.
Science 1999 Apr; 285(5414):662.
-
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.
Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG Jr, Elledge SJ, Conaway RC, Harper JW, Conaway JW.
Science 1999 Apr; 285(5414):657.
-
Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.
Tan P, Fuchs SY, Chen A, Wu K, Gomez C, Ronai Z, Pan ZQ.
Molecular Cell 1999 Apr; 3(4):527.
-
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y.
Molecular Cell 1999 Apr; 3(4):535.
-
The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com