Western blot using RNF2 polyclonal antibody (Cat # PAB10232) shows detection of a 38 KDa band corresponding to human RNF2 in 3T3 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), mouse brain (Lane 4) and CHO-K1 (Lane 5) cell lysates. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature. Signal was detected using standard techniques.
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq