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Last updated: 2016/12/4
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RNF2 polyclonal antibody

  • Catalog # : PAB10232
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  • Specification
  • Product Description:
  • goat polyclonal antibody raised against synthetic peptide of RNF2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 189-201 of human RNF2.
  • Host:
  • Goat
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Orangutan, Rat
  • Specificity:
  • This affinity purified antibody is directed against human RING1B protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using RNF2 polyclonal antibody (Cat # PAB10232) shows detection of a 38 KDa band corresponding to human RNF2 in 3T3 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), mouse brain (Lane 4) and CHO-K1 (Lane 5) cell lysates.
    Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature.
    Signal was detected using standard techniques.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6045
  • Protein Accession#:
  • Q99496;NP_009143
  • Gene Name:
  • RNF2
  • Gene Alias:
  • BAP-1,BAP1,DING,HIPI3,RING1B,RING2
  • Gene Description:
  • ring finger protein 2
  • Gene Summary:
  • Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033405,OTTHUMP00000060668
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