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Last updated: 2017/11/19
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CBX4 polyclonal antibody

  • Catalog # : PAB10228
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CBX4.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 95-107 of human CBX4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • Reactivity occurs against human PC2 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:1000-1:4000)
    Immunofluorescence (1:100-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blotting analysis shows the detection of human CBX4 in probed lysates using CBX4 polyclonal antibody (Cat # PAB10228).
    The panel on the left shows the blot probed with CBX4 polyclonal antibody.
    The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate.
    In the left panel the band labeled as CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
    The bands labeled with stars are likely endogenous CBX4.
    In the right panel the band labeled CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
    Data contributed by Dr. Ari Melnick, Albert Einstein College of Medicine.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy of CBX4 polyclonal antibody (Cat # PAB10228) was used for immunofluorescent imaging of U-2 OS.
    The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of CBX4 (see Satijn et al. below) .
    Primary antibody used at 1 : 200.
    Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8535
  • Protein Accession#:
  • GC17M081095(Human);LocusLink8535(Human);Omim603079(Human);SwissProtO00257(Human);Unigene5637(Human)
  • Gene Name:
  • CBX4
  • Gene Alias:
  • NBP16,PC2,hPC2
  • Gene Description:
  • chromobox homolog 4 (Pc class homolog, Drosophila)
  • Other Designations:
  • NS5ATP1-binding protein 16,chromobox homolog 4
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