Western blotting analysis shows the detection of human CBX4 in probed lysates using CBX4 polyclonal antibody (Cat # PAB10228). The panel on the left shows the blot probed with CBX4 polyclonal antibody. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate. In the left panel the band labeled as CBX4 is FLAG-tagged transfected CBX4 in 293T cells. The bands labeled with stars are likely endogenous CBX4. In the right panel the band labeled CBX4 is FLAG-tagged transfected CBX4 in 293T cells. Data contributed by Dr. Ari Melnick, Albert Einstein College of Medicine.
Immunofluorescence microscopy of CBX4 polyclonal antibody (Cat # PAB10228) was used for immunofluorescent imaging of U-2 OS. The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of CBX4 (see Satijn et al. below) . Primary antibody used at 1 : 200. Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.