CBX4 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blotting analysis shows the detection of human CBX4 in probed lysates using CBX4 polyclonal antibody (Cat # PAB10228).
The panel on the left shows the blot probed with CBX4 polyclonal antibody.
The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate.
In the left panel the band labeled as CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
The bands labeled with stars are likely endogenous CBX4.
In the right panel the band labeled CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
Data contributed by Dr. Ari Melnick, Albert Einstein College of Medicine.
Immunofluorescence
Immunofluorescence microscopy of CBX4 polyclonal antibody (Cat # PAB10228) was used for immunofluorescent imaging of U-2 OS.
The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of CBX4 (see Satijn et al. below) .
Primary antibody used at 1 : 200.
Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CBX4.
Immunogen
A synthetic peptide corresponding to amino acids 95-107 of human CBX4.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
Reactivity occurs against human PC2 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:1000-1:4000)
Immunofluorescence (1:100-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blotting analysis shows the detection of human CBX4 in probed lysates using CBX4 polyclonal antibody (Cat # PAB10228).
The panel on the left shows the blot probed with CBX4 polyclonal antibody.
The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate.
In the left panel the band labeled as CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
The bands labeled with stars are likely endogenous CBX4.
In the right panel the band labeled CBX4 is FLAG-tagged transfected CBX4 in 293T cells.
Data contributed by Dr. Ari Melnick, Albert Einstein College of Medicine.Immunofluorescence
Immunofluorescence microscopy of CBX4 polyclonal antibody (Cat # PAB10228) was used for immunofluorescent imaging of U-2 OS.
The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of CBX4 (see Satijn et al. below) .
Primary antibody used at 1 : 200.
Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBX4
Entrez GeneID
8535Protein Accession#
GC17M081095(Human);LocusLink8535(Human);Omim603079(Human);SwissProtO00257(Human);Unigene5637(Human)Gene Name
CBX4
Gene Alias
NBP16, PC2, hPC2
Gene Description
chromobox homolog 4 (Pc class homolog, Drosophila)
Omim ID
603079Gene Ontology
HyperlinkGene Summary
O
Other Designations
NS5ATP1-binding protein 16|chromobox homolog 4
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Interactome
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Publication Reference
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The polycomb protein Pc2 is a SUMO E3.
Kagey MH, Melhuish TA, Wotton D.
Cell 2003 Apr; 113(1):127.
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Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
Satijn DP, Olson DJ, van der Vlag J, Hamer KM, Lambrechts C, Masselink H, Gunster MJ, Sewalt RG, van Driel R, Otte AP.
Molecular and Cellular Biology 1997 Oct; 17(10):6076.
Application：IF, WB-Ce, WB-Tr, Human, U2OS, C57MG cells.
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RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
Satijn DP, Gunster MJ, van der Vlag J, Hamer KM, Schul W, Alkema MJ, Saurin AJ, Freemont PS, van Driel R, Otte AP.
Molecular and Cellular Biology 1997 Jul; 17(7):4105.
Application：IF, IP, WB-Ce, Human, Saos-2, SW480 cells.
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The polycomb protein Pc2 is a SUMO E3.
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