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Last updated: 2016/12/4
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NIPBL polyclonal antibody

  • Catalog # : PAB10226
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NIPBL.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 344-356 of human NIPBL.
  • Host:
  • Goat
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Orangutan, Rat
  • Specificity:
  • This affinity purified antibody is directed against human IDN3 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of bands at ~315 KDa and ~125 KDa corresponding to isoforms of NIPBL (arrow-heads) in mouse heart whole cell tissue extract.
    Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1,500.
    Reaction occurredovernight at 4°C followed by washes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
    IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trade mark of LI-COR, Inc.
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of a band ~300 KDa correspondingto NIPBL (arrowhead) in human kidney (lane1) and human heart (Lane 2) whole cell tissue extracts.
    Approximately 20 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:500.
    Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q6KC79;BAA77335
  • Gene Name:
  • NIPBL
  • Gene Alias:
  • CDLS,CDLS1,DKFZp434L1319,FLJ11203,FLJ12597,FLJ13354,FLJ13648,FLJ44854,IDN3,IDN3-B
  • Gene Description:
  • Nipped-B homolog (Drosophila)
  • Gene Summary:
  • This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000116005,SCC2 homolog,delangin,nipped-B-like
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