Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of bands at ~315 KDa and ~125 KDa corresponding to isoforms of NIPBL (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurredovernight at 4°C followed by washes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trade mark of LI-COR, Inc.
Western Blot (Tissue lysate)
Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of a band ~300 KDa correspondingto NIPBL (arrowhead) in human kidney (lane1) and human heart (Lane 2) whole cell tissue extracts. Approximately 20 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.
This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq