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Last updated: 2017/1/15
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USP7 polyclonal antibody

  • Catalog # : PAB10225
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of USP7.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human USP7.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:15000-1:60000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using USP7 polyclonal antibody (Cat # PAB10225) shows detection of USP7 in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A-431, Lane 4 - MCF-7, Lane 5 - 3T3).
    The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.
    A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.
    Exposure time was 30 sec.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7874
  • Protein Accession#:
  • NP_003461;Q93009
  • Gene Name:
  • USP7
  • Gene Alias:
  • HAUSP,TEF1
  • Gene Description:
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • Other Designations:
  • Herpes virus-associated ubiquitin-specific protease,ubiquitin specific protease 7,ubiquitin specific protease 7 (herpes virus-associated)
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