USP7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using USP7 polyclonal antibody (Cat # PAB10225) shows detection of USP7 in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A-431, Lane 4 - MCF-7, Lane 5 - 3T3).
The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.
A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.
Exposure time was 30 sec.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP7.
Immunogen
A synthetic peptide corresponding to N-terminus of human USP7.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:60000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using USP7 polyclonal antibody (Cat # PAB10225) shows detection of USP7 in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A-431, Lane 4 - MCF-7, Lane 5 - 3T3).
The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.
A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.
Exposure time was 30 sec.Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP7
Entrez GeneID
7874Protein Accession#
NP_003461;Q93009Gene Name
USP7
Gene Alias
HAUSP, TEF1
Gene Description
ubiquitin specific peptidase 7 (herpes virus-associated)
Omim ID
602519Gene Ontology
HyperlinkOther Designations
Herpes virus-associated ubiquitin-specific protease|ubiquitin specific protease 7|ubiquitin specific protease 7 (herpes virus-associated)
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Interactome
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Disease
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Publication Reference
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Molecular recognition of p53 and MDM2 by USP7/HAUSP.
Sheng Y, Saridakis V, Sarkari F, Duan S, Wu T, Arrowsmith CH, Frappier L.
Nature Structural & Molecular Biology 2006 Mar; 13(3):285.
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Reciprocal activities between herpes simplex virus type 1 regulatory protein ICP0, a ubiquitin E3 ligase, and ubiquitin-specific protease USP7.
Boutell C, Canning M, Orr A, Everett RD.
Journal of Virology 2005 Oct; 79(19):12342.
Application：WB-Tr, Human, HeLa cells.
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Expression and functional analyses of mHAUSP regulating apoptosis of cervical adenocarcinoma cells.
Yoo KJ, Lee HJ, Lee H, Lee KY, Lee SH, Chung HM, Baek KH.
International Journal of Oncology 2005 Jul; 27(1):97.
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Molecular recognition of p53 and MDM2 by USP7/HAUSP.
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