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Last updated: 2017/12/10
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ATAD5 polyclonal antibody

  • Catalog # : PAB10222
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ATAD5.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 63-76 of human ATAD5.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human
  • Specificity:
  • The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:15000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates.
    Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates.
    After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.
    The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.
    The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa.
    The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
    Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • Q96QE3;CAC44537
  • Gene Name:
  • ATAD5
  • Gene Alias:
  • C17orf41,FLJ12735,FRAG1
  • Gene Description:
  • ATPase family, AAA domain containing 5
  • Other Designations:
  • chromosome fragility associated gene 1
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