ATAD5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates.
Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates.
After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.
The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.
The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa.
The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATAD5.
Immunogen
A synthetic peptide corresponding to amino acids 63-76 of human ATAD5.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human
Specificity
The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:3000-1:15000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates.
Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates.
After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.
The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.
The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa.
The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATAD5
Entrez GeneID
79915Protein Accession#
Q96QE3;CAC44537Gene Name
ATAD5
Gene Alias
C17orf41, FLJ12735, FRAG1
Gene Description
ATPase family, AAA domain containing 5
Omim ID
609534Gene Ontology
HyperlinkGene Summary
O
Other Designations
chromosome fragility associated gene 1
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Interactome
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Disease
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Publication Reference
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Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability.
Kanellis P, Agyei R, Durocher D.
Current Biology 2003 Sep; 13(18):1583.
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A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions.
Jenne DE, Tinschert S, Stegmann E, Reimann H, Nurnberg P, Horn D, Naumann I, Buske A, Thiel G.
Genomics 2000 May; 66(1):93.
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Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability.
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