Rabbit polyclonal antibody raised against synthetic peptide of ATAD5.
A synthetic peptide corresponding to amino acids 63-76 of human ATAD5.
Chimpanzee, Dog, Human
The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:3000-1:15000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates. Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time. The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa. The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).