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Last updated: 2016/10/23
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AP1G1 polyclonal antibody

  • Catalog # : PAB10221
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of AP1G1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 646-659 of human AP1G1.
  • Host:
  • Goat
  • Reactivity:
  • Chimpanzee, Human, Orangutan
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:8000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1).
    Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2).
    Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody.
    Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection using a 60-sec exposure time.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 164
  • Protein Accession#:
  • O43747;NP_001119
  • Gene Name:
  • AP1G1
  • Gene Alias:
  • ADTG,CLAPG1,MGC18255
  • Gene Description:
  • adaptor-related protein complex 1, gamma 1 subunit
  • Gene Summary:
  • Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • clathrin assembly protein complex 1 gamma large chain,clathrin-associated/assembly/adaptor protein, large, gamma 1,gamma adaptin,golgi adaptor HA1/AP1 adaptin gamma subunit
  • Gene Pathway
  • Related Disease
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