AP1G1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1).
Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2).
Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 60-sec exposure time.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AP1G1.
Immunogen
A synthetic peptide corresponding to amino acids 646-659 of human AP1G1.
Host
Goat
Reactivity
Chimpanzee, Human, Orangutan
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:8000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1).
Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2).
Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 60-sec exposure time.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AP1G1
Entrez GeneID
164Protein Accession#
O43747;NP_001119Gene Name
AP1G1
Gene Alias
ADTG, CLAPG1, MGC18255
Gene Description
adaptor-related protein complex 1, gamma 1 subunit
Omim ID
603533Gene Ontology
HyperlinkGene Summary
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
clathrin assembly protein complex 1 gamma large chain|clathrin-associated/assembly/adaptor protein, large, gamma 1|gamma adaptin|golgi adaptor HA1/AP1 adaptin gamma subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.
Nogi T, Shiba Y, Kawasaki M, Shiba T, Matsugaki N, Igarashi N, Suzuki M, Kato R, Takatsu H, Nakayama K, Wakatsuki S.
Nature Structural Biology 2002 Jul; 9(7):527.
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Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain.
Shiba Y, Takatsu H, Shin HW, Nakayama K.
Journal of Biochemistry 2002 Mar; 131(3):327.
Application：IF, WB-Tr, Human, HeLa cells.
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Identification and characterization of novel clathrin adaptor-related proteins.
Takatsu H, Sakurai M, Shin HW, Murakami K, Nakayama K.
The Journal of Biological Chemistry 1998 Sep; 273(38):24693.
Application：IF, WB-Tr, Human, Rat, HEK 293, HL-60, Rat hepatocyte Clone 9 cells cells.
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Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.
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