Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1). Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 60-sec exposure time.
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
clathrin assembly protein complex 1 gamma large chain,clathrin-associated/assembly/adaptor protein, large, gamma 1,gamma adaptin,golgi adaptor HA1/AP1 adaptin gamma subunit