HSP90AA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10219) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in an SK-BR-3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor).
Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA).
Immuno-precipitation with HSP90AA1 polyclonal antibody was performed prior to immunoblotting with anti-HSP90AA1 acetyl K294.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HSP90AA1 polyclonal antibody (Cat # PAB10219) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1.
Immunogen
A synthetic peptide corresponding to residues surrounding K294 of human HSP90AA1.
Host
Rabbit
Reactivity
Chicken, Fruit fly, Human, Monkey, Mouse, Rat
Specificity
This affinity purified antibody is directed against human Hsp90 protein acetylated at K294.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2500-1:10000)
Western Blot (1:500-1:2500)
Immunohistochemistry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10219) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in an SK-BR-3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor).
Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA).
Immuno-precipitation with HSP90AA1 polyclonal antibody was performed prior to immunoblotting with anti-HSP90AA1 acetyl K294.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HSP90AA1 polyclonal antibody (Cat # PAB10219) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSP90AA1
Entrez GeneID
3320Protein Accession#
P46633;P07900Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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An acetylation site in the middle domain of Hsp90 regulates chaperone function.
Scroggins BT, Robzyk K, Wang D, Marcu MG, Tsutsumi S, Beebe K, Cotter RJ, Felts S, Toft D, Karnitz L, Rosen N, Neckers L.
Molecular Cell 2007 Jan; 25(1):151.
Application：IP, Human, SK-BR-3 cells.
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HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor.
Kovacs JJ, Murphy PJ, Gaillard S, Zhao X, Wu JT, Nicchitta CV, Yoshida M, Toft DO, Pratt WB, Yao TP.
Molecular Cell 2005 May; 18(5):601.
Application：IP, WB, Human, A-549 cells.
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Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.
Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K.
Molecular Cancer Therapeutics 2003 Oct; 2(10):971.
Application：IP-WB, WB-Ce, Human, SKBR-3, BT-474 cells.
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An acetylation site in the middle domain of Hsp90 regulates chaperone function.
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