HSP90AA1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10218) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in various lysate preparations (arrowhead).
COS-7 transfected with mutant K294R HSP90AA1 (left two lanes), COS-7 transfected with wild type HSP90AA1 (middle two lanes) and empty vector CSO-7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated.
Transfected cells express either mutant or wt HSP90AA1 coupled to Flag™tag.
Anti-Flag™ immunoprecipitation was performed prior to immunoblotting with HSP90AA1 polyclonal antibody.
Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1.
Immunogen
A synthetic peptide corresponding to amino acids 289-300 of human HSP90AA1.
Host
Rabbit
Reactivity
Chicken, Fruit fly, Human, Monkey, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10218) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in various lysate preparations (arrowhead).
COS-7 transfected with mutant K294R HSP90AA1 (left two lanes), COS-7 transfected with wild type HSP90AA1 (middle two lanes) and empty vector CSO-7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated.
Transfected cells express either mutant or wt HSP90AA1 coupled to Flag™tag.
Anti-Flag™ immunoprecipitation was performed prior to immunoblotting with HSP90AA1 polyclonal antibody.
Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSP90AA1
Entrez GeneID
3320Protein Accession#
P46633;P07900Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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An acetylation site in the middle domain of Hsp90 regulates chaperone function.
Scroggins BT, Robzyk K, Wang D, Marcu MG, Tsutsumi S, Beebe K, Cotter RJ, Felts S, Toft D, Karnitz L, Rosen N, Neckers L.
Molecular Cell 2007 Jan; 25(1):151.
Application：IP, Human, SK-BR-3 cells.
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HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor.
Kovacs JJ, Murphy PJ, Gaillard S, Zhao X, Wu JT, Nicchitta CV, Yoshida M, Toft DO, Pratt WB, Yao TP.
Molecular Cell 2005 May; 18(5):601.
Application：IP, WB, Human, A-549 cells.
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Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.
Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K.
Molecular Cancer Therapeutics 2003 Oct; 2(10):971.
Application：IP-WB, WB-Ce, Human, SKBR-3, BT-474 cells.
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An acetylation site in the middle domain of Hsp90 regulates chaperone function.
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