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Last updated: 2016/12/4

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Fap polyclonal antibody

  • Catalog # : PAB10209
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Fap.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of mouse Fap.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is known to react with mouse FAP protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:100000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using Fap polyclonal antibody (Cat # PAB10209) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells (Lane 4), but not control HEK cells (Lane 3).
    Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).
    The band at ~90 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP.
    Primary antibody was used at 1:1,000.
    Personal communication, S.Kim, NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_032012;P97321
  • Gene Name:
  • Fap
  • Gene Alias:
  • -
  • Gene Description:
  • fibroblast activation protein
  • Other Designations:
  • OTTMUSP00000013791
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