Fap polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using Fap polyclonal antibody (Cat # PAB10209) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells (Lane 4), but not control HEK cells (Lane 3).
Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).
The band at ~90 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP.
Primary antibody was used at 1:1,000.
Personal communication, S.Kim, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Fap.
Immunogen
A synthetic peptide corresponding to internal region of mouse Fap.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is known to react with mouse FAP protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using Fap polyclonal antibody (Cat # PAB10209) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells (Lane 4), but not control HEK cells (Lane 3).
Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).
The band at ~90 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP.
Primary antibody was used at 1:1,000.
Personal communication, S.Kim, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Fap
Entrez GeneID
14089Protein Accession#
NP_032012;P97321Gene Name
Fap
Gene Alias
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Gene Description
fibroblast activation protein
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000013791
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Publication Reference
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Abrogation of fibroblast activation protein enzymatic activity attenuates tumor growth.
Cheng JD, Valianou M, Canutescu AA, Jaffe EK, Lee HO, Wang H, Lai JH, Bachovchin WW, Weiner LM.
Molecular Cancer Therapeutics 2005 Mar; 4(3):351.
Application：IHC-P, WB, Human, HEK 293, HT-29 cells.
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FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.
Ramirez-Montagut T, Blachere NE, Sviderskaya EV, Bennett DC, Rettig WJ, Garin-Chesa P, Houghton AN.
Oncogene 2004 Jul; 23(32):5435.
Application：Flow Cyt, Mouse, MEFs, Mouse melanocytes, Thymocytes, Tumors.
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Expression of the fibroblast activation protein during mouse embryo development.
Niedermeyer J, Garin-Chesa P, Kriz M, Hilberg F, Mueller E, Bamberger U, Rettig WJ, Schnapp A.
The International Journal of Developmental Biology 2001 Apr; 45(2):445.
Application：IP, Mouse, Embryos.
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Abrogation of fibroblast activation protein enzymatic activity attenuates tumor growth.
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