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Matrix Protein p15 polyclonal antibody

  • Catalog # : PAB10208
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Matrix Protein p15.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of Feline Immunodeficiency Virus Matrix Protein p15.
  • Host:
  • Rabbit
  • Specificity:
  • This affinity purified antibody is directed against Feline Immunodeficiency Virus (FIV) Matrix Protein p15.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:1000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using Matrix Protein p15 polyclonal antibody (Cat # PAB10208) to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (Lane 2, arrowhead).
    Lane 1 is an uninfected control.
    Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose.
    The membrane was probed with the primary antibody at a 1:10,000 dilution.
    Personal Communication, B. Luttge, CCR-NCI, Frederick, MD.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
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