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Last updated: 2016/12/4
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FBXO5 polyclonal antibody

  • Catalog # : PAB10207
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FBXO5.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human FBXO5.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using FBXO5 polyclonal antibody (Cat # PAB10207) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate (Lane 1 arrowhead).
    The identity of cross-reactive minor bands at ~72 KDa and 150 KDa is unknown.
    Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).
    Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.
    The membrane was blocked using BLOTTO.
    Primary antibody was used at a 1:500 dilution in BLOTTO.
    The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of FBXO5 polyclonal antibody (Cat # PAB10207) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_036309;Q9UKT4
  • Gene Name:
  • FBXO5
  • Gene Alias:
  • EMI1,FBX5,Fbxo31
  • Gene Description:
  • F-box protein 5
  • Gene Summary:
  • This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • F-box only protein 5,F-box protein Fbx5,OTTHUMP00000017453,early mitotic inhibitor 1
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