FBXO5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using FBXO5 polyclonal antibody (Cat # PAB10207) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate (Lane 1 arrowhead).
The identity of cross-reactive minor bands at ~72 KDa and 150 KDa is unknown.
Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).
Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.
The membrane was blocked using BLOTTO.
Primary antibody was used at a 1:500 dilution in BLOTTO.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of FBXO5 polyclonal antibody (Cat # PAB10207) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FBXO5.
Immunogen
A synthetic peptide corresponding to N-terminus of human FBXO5.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using FBXO5 polyclonal antibody (Cat # PAB10207) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate (Lane 1 arrowhead).
The identity of cross-reactive minor bands at ~72 KDa and 150 KDa is unknown.
Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).
Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.
The membrane was blocked using BLOTTO.
Primary antibody was used at a 1:500 dilution in BLOTTO.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of FBXO5 polyclonal antibody (Cat # PAB10207) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXO5
Entrez GeneID
26271Protein Accession#
NP_036309;Q9UKT4Gene Name
FBXO5
Gene Alias
EMI1, FBX5, Fbxo31
Gene Description
F-box protein 5
Omim ID
606013Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
F-box only protein 5|F-box protein Fbx5|OTTHUMP00000017453|early mitotic inhibitor 1
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Interactome
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Publication Reference
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Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
Hansen DV, Loktev AV, Ban KH, Jackson PK.
Molecular Biology of the Cell 2004 Dec; 15(12):5623.
Application：IF, WB-Tr, Human, HeLa , U2OScells.
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E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1).
Hsu JY, Reimann JD, Sørensen CS, Lukas J, Jackson PK.
Nature Cell Biology 2002 May; 4(5):358.
Application：IF, IP, WB-Ce, WB-Tr, Human, BJ fibroblasts, HeLa cells.
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Emi1 is required for cytostatic factor arrest in vertebrate eggs.
Reimann JD, Jackson PK.
Nature 2002 Apr; 416(6883):850.
Application：WB, WB-Tr, Xenopus, Oocytes, CSF.
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Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
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