Western blot using FBXO5 polyclonal antibody (Cat # PAB10207) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate (Lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 KDa and 150 KDa is unknown. Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2). Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:500 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).
Immunohistochemistry of FBXO5 polyclonal antibody (Cat # PAB10207) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
F-box only protein 5,F-box protein Fbx5,OTTHUMP00000017453,early mitotic inhibitor 1