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Last updated: 2016/12/4
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FBXO43 polyclonal antibody

  • Catalog # : PAB10206
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FBXO43.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus 60 amino acids of human FBXO43.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human
  • Specificity:
  • This affinity-purified antibody is directed against human Fbp5B protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using FBXO43 polyclonal antibody (Cat # PAB10206).
    Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate.
    Panel A shows antibody reactivity.
    Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.
    This pre-incubation greatly diminishes specific band recognition.
    Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.
    When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown).
    Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_001025031;Q4G163
  • Gene Name:
  • FBXO43
  • Gene Alias:
  • EMI2,ERP1,Fbx43
  • Gene Description:
  • F-box protein 43
  • Gene Summary:
  • Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
  • Other Designations:
  • early mitotic inhibitor 2
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