FBXO43 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using FBXO43 polyclonal antibody (Cat # PAB10206).
Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate.
Panel A shows antibody reactivity.
Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.
This pre-incubation greatly diminishes specific band recognition.
Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.
When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown).
Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FBXO43.
Immunogen
A synthetic peptide corresponding to N-terminus 60 amino acids of human FBXO43.
Host
Rabbit
Reactivity
Chimpanzee, Human
Specificity
This affinity-purified antibody is directed against human Fbp5B protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using FBXO43 polyclonal antibody (Cat # PAB10206).
Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate.
Panel A shows antibody reactivity.
Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.
This pre-incubation greatly diminishes specific band recognition.
Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.
When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown).
Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXO43
Entrez GeneID
286151Protein Accession#
NP_001025031;Q4G163Gene Name
FBXO43
Gene Alias
EMI2, ERP1, Fbx43
Gene Description
F-box protein 43
Omim ID
609110Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
early mitotic inhibitor 2
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Interactome
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Publication Reference
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Systematic analysis and nomenclature of mammalian F-box proteins.
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW.
Genes & Development 2004 Nov; 18(21):2573.
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Systematic analysis and nomenclature of mammalian F-box proteins.
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