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Last updated: 2017/12/10
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FBX4 polyclonal antibody

  • Catalog # : PAB10205
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FBX4.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human and mouse FBX4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antiserum is directed against human and mouse FBX4.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Whole cell extracts prepared from U-2 OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose.
    FBX4 was detected using FBX4 polyclonal antibody (Cat # PAB10205) at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG.
    Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of NIH/3T3 cells with FBX4 polyclonal antibody (Cat # PAB10205) . Cells were fixed in methanol/acetone followed by incubation with FBX4 polyclonal antibody at a 1 : 300 dilution.
    Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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