Eif3e polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e in whole cell extracts from murine (HC-11 and NIH/3T3), monkey (CV-1 and COS-1), and human (HEK293T) cell lines as well as over-expressed Eif3e (control transfected flag-tagged Eif3e). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 KDa, indicated by the arrowhead, corresponds to flag-tagged Eif3e.
Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
Western Blot (Recombinant protein)
Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
Personal communication, J.Lee, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Eif3e.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Eif3e.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Monkey, Mouse, Rat
Specificity
This antibody is specific to mouse eIF3S6/Int6 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:90000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e in whole cell extracts from murine (HC-11 and NIH/3T3), monkey (CV-1 and COS-1), and human (HEK293T) cell lines as well as over-expressed Eif3e (control transfected flag-tagged Eif3e). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 KDa, indicated by the arrowhead, corresponds to flag-tagged Eif3e.
Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.Western Blot (Recombinant protein)
Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
Personal communication, J.Lee, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Eif3e
Entrez GeneID
16341Protein Accession#
NP_032414;P60229Gene Name
Eif3e
Gene Alias
48kDa, Eif3s6, Int6, eIF3-p46, eIF3-p48
Gene Description
eukaryotic translation initiation factor 3, subunit E
Gene Ontology
HyperlinkGene Summary
O
Other Designations
eukaryotic translation initiation factor 3, subunit 6|eukaryotic translation initiation factor 3, subunit 6 48kDa|mammary tumor integration site 6
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Publication Reference
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A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences.
Lowther W, Wiley K, Smith GH, Callahan R.
Journal of Virology 2005 Aug; 79(15):10093.
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Int6 expression can predict survival in early-stage non-small cell lung cancer patients.
Buttitta F, Martella C, Barassi F, Felicioni L, Salvatore S, Rosini S, D'Antuono T, Chella A, Mucilli F, Sacco R, Mezzetti A, Cuccurullo F, Callahan R, Marchetti A.
Clinical Cancer Research 2005 May; 11(9):3198.
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Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation.
Bandyopadhyay A, Matsumoto T, Maitra U.
Molecular Biology of the Cell 2000 Nov; 11(11):4005.
Application：WB-Ce, Rabbit, Reticulocyte.
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A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences.
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