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Last updated: 2017/10/22

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Eif3e polyclonal antibody

  • Catalog # : PAB10203
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Eif3e.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of mouse Eif3e.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Monkey, Mouse, Rat
  • Specificity:
  • This antibody is specific to mouse eIF3S6/Int6 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:90000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e in whole cell extracts from murine (HC-11 and NIH/3T3), monkey (CV-1 and COS-1), and human (HEK293T) cell lines as well as over-expressed Eif3e (control transfected flag-tagged Eif3e). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 KDa, indicated by the arrowhead, corresponds to flag-tagged Eif3e.
    Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using Eif3e polyclonal antibody (Cat # PAB10203) shows detection of endogenous Eif3e. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
    Personal communication, J.Lee, NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_032414;P60229
  • Gene Name:
  • Eif3e
  • Gene Alias:
  • 48kDa,Eif3s6,Int6,eIF3-p46,eIF3-p48
  • Gene Description:
  • eukaryotic translation initiation factor 3, subunit E
  • Other Designations:
  • eukaryotic translation initiation factor 3, subunit 6,eukaryotic translation initiation factor 3, subunit 6 48kDa,mammary tumor integration site 6
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