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Last updated: 2016/12/4
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DIAPH2 polyclonal antibody

  • Catalog # : PAB10201
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DIAPH2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1085-1101 of human DIAPH2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:12000-1:60000)
    Western Blot (1:1000-1:6000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using DIAPH2 polyclonal antibody (Cat # PAB10201) shows detection of a 132 KDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.
    Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.
    Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
    Achemiluminescence system was used forsignal detection using a 1 min exposure time.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1730
  • Protein Accession#:
  • O60879;NP_006720
  • Gene Name:
  • DIAPH2
  • Gene Alias:
  • DIA,DIA2,DRF2,FLJ11167,POF,POF2
  • Gene Description:
  • diaphanous homolog 2 (Drosophila)
  • Gene Summary:
  • The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000024270,OTTHUMP00000062171,diaphanous 2,diaphanous-related formin-2
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