Western blot using DIAPH2 polyclonal antibody (Cat # PAB10201) shows detection of a 132 KDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. Achemiluminescence system was used forsignal detection using a 1 min exposure time.
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq