DLL4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot using DLL4 polyclonal antibody (Cat # PAB10200) shows detection of a 74 KDa band corresponding to DLL4 in a lysate prepared from mouse pancreatic tissue.
Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DLL4 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 3 min exposure time.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DLL4 polyclonal antibody (Cat # PAB10200) was used at 20 ug/mL to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus.
In contrast to reported findings, no staining was observed in vascular tissue.
This image shows DLL4 staining of human ovary.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DLL4.
Immunogen
A synthetic peptide corresponding to internal region of human DLL4.
Host
Rabbit
Reactivity
Chimpanzee, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against human DELTA-4.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:2500)
Immunohistochemistry (10-20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using DLL4 polyclonal antibody (Cat # PAB10200) shows detection of a 74 KDa band corresponding to DLL4 in a lysate prepared from mouse pancreatic tissue.
Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DLL4 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 3 min exposure time.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DLL4 polyclonal antibody (Cat # PAB10200) was used at 20 ug/mL to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus.
In contrast to reported findings, no staining was observed in vascular tissue.
This image shows DLL4 staining of human ovary.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DLL4
Entrez GeneID
54567Protein Accession#
Q9NR61;NP_061947Gene Name
DLL4
Gene Alias
MGC126344, hdelta2
Gene Description
delta-like 4 (Drosophila)
Omim ID
605185Gene Ontology
HyperlinkGene Summary
This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq
Other Designations
delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|notch ligand DLL4|notch ligand delta-2
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Pathway
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Publication Reference
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Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast.
Plessl K, Haider S, Fiala C, Pollheimer J, Knofler M.
Placenta 2015 Apr; 36(4):365.
Application：IF, Human, Placental, Decidual.
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Membrane-bound delta-4 notch ligand reduces the proliferative activity of primitive human hematopoietic CD34+CD38low cells while maintaining their LTC-IC potential.
Lauret E, Catelain C, Titeux M, Poirault S, Dando JS, Dorsch M, Villeval JL, Groseil A, Vainchenker W, Sainteny F, Bennaceur-Griscelli A.
Leukemia 2004 Apr; 18(4):788.
Application：Flow Cyt, Mouse, S17 cells.
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Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines.
Tohda S, Murata-Ohsawa M, Sakano S, Nara N.
International Journal of Oncology 2003 May; 22(5):1073.
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Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.
Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M.
Molecular and Cellular Biology 2003 Jan; 23(1):14.
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Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast.
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