Western blot using DLL4 polyclonal antibody (Cat # PAB10200) shows detection of a 74 KDa band corresponding to DLL4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DLL4 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 3 min exposure time.
Immunohistochemistry of DLL4 polyclonal antibody (Cat # PAB10200) was used at 20 ug/mL to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows DLL4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.