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Last updated: 2017/10/22
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DLL4 polyclonal antibody

  • Catalog # : PAB10200
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DLL4.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human DLL4.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human DELTA-4.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2500)
    Immunohistochemistry (10-20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using DLL4 polyclonal antibody (Cat # PAB10200) shows detection of a 74 KDa band corresponding to DLL4 in a lysate prepared from mouse pancreatic tissue.
    Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DLL4 polyclonal antibody.
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection using a 3 min exposure time.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of DLL4 polyclonal antibody (Cat # PAB10200) was used at 20 ug/mL to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus.
    In contrast to reported findings, no staining was observed in vascular tissue.
    This image shows DLL4 staining of human ovary.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9NR61;NP_061947
  • Gene Name:
  • DLL4
  • Gene Alias:
  • MGC126344,hdelta2
  • Gene Description:
  • delta-like 4 (Drosophila)
  • Gene Summary:
  • This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq
  • Other Designations:
  • delta 4,delta ligand 4,delta-like 4 homolog,delta-like 4 protein,notch ligand DLL4,notch ligand delta-2
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