Ccne2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using Ccne2 polyclonal antibody (Cat # PAB10197) shows specific detection of Ccne2.
Cell extracts over-expressing mouse Ccne1 (Lane 1) and Ccne2 (Lane 2) were electrophoresed, transferred to nitrocellulose, and probed with theCcne2 polyclonal antibody.
The affinity purified antibody also detects endogenous Ccne2 in Skp2 -/- MEF cells. (data not shown).
Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ccne2.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Ccne2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Cyclin E2 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:85000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using Ccne2 polyclonal antibody (Cat # PAB10197) shows specific detection of Ccne2.
Cell extracts over-expressing mouse Ccne1 (Lane 1) and Ccne2 (Lane 2) were electrophoresed, transferred to nitrocellulose, and probed with theCcne2 polyclonal antibody.
The affinity purified antibody also detects endogenous Ccne2 in Skp2 -/- MEF cells. (data not shown).
Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ccne2
Entrez GeneID
12448Protein Accession#
AAC80527;Q9Z238Gene Name
Ccne2
Gene Alias
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Gene Description
cyclin E2
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000004878|OTTMUSP00000004879
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Publication Reference
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Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps.
Amulic B, Knackstedt SL, Abu Abed U, Deigendesch N, Harbort CJ, Caffrey BE, Brinkmann V, Heppner FL, Hinds PW, Zychlinsky A.
Developmental Cell 2017 Nov; 43(4):449.
Application：WB-Ce, Human , Human Peripheral Blood Neutrophils.
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Cdc2-cyclin E complexes regulate the G1/S phase transition.
Aleem E, Kiyokawa H, Kaldis P.
Nature Cell Biology 2005 Aug; 7(8):831.
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Mammalian cell cycles without cyclin E-CDK2.
Yu Q, Sicinski P.
Cell Cycle 2004 Mar; 3(3):292.
Application：WB, Human, Human mammalian cells.
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Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells.
Parisi T, Beck AR, Rougier N, McNeil T, Lucian L, Werb Z, Amati B.
The EMBO Journal 2003 Sep; 22(18):4794.
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Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps.
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