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Last updated: 2016/10/23
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Collagen Type I polyclonal antibody

  • Catalog # : PAB10190
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against native Collagen Type I.
  • Immunogen:
  • Native purified human and bovine placenta Collagen Type I.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Human
  • Specificity:
  • This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Native Purified Protein.
  • Recommend Usage:
  • ELISA (1:5000-1:50000)
    Western Blot (1:5000-1:50000)
    Immunohistochemistry (1:50-1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 100 mM Na2B4O7, 75 mM NaCl, 5 mM EDTA, pH 8.0 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After dilution, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis is shown using Collagen Type I polyclonal antibody (Cat # PAB10190) to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARgamma-transduced cell lysates (right lane).
    Protein staining shown below each blot depicts equal protein loading.
    An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes.
    Proteins were detected by incubating the membrane with Collagen Type I polyclonal antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk.
    Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 ug/10 ml.
    Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).
    See Hazra et al. (2004) for additional details.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with Collagen Type I polyclonal antibody (Cat # PAB10190) was used at a 1 : 100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
    Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
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