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Last updated: 2017/8/20

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Myb polyclonal antibody

  • Catalog # : PAB10189
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Myb.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of mouse Myb.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Gorilla, Horse, Human, Monkey, Mouse, Opossum, Orangutan, Pig, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:25000)
    Western Blot (1:250-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using Myb polyclonal antibody (Cat # PAB10189) in COS-7 cell lysates.
    Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce Myb modification.
    Lane 3 shows detection of bands at 75, 98, and 125 KDa corresponding to overexpressed Myb (arrows) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
    Nitrocellulose 0.45 um membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS.
    The primary antibody was used at 1:500.
    Personal communication, J. Bies, NCI NIH, Bethesda.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_034978;P06876
  • Gene Name:
  • Myb
  • Gene Alias:
  • AI550390,M16449,MGC18531,c-myb
  • Gene Description:
  • myeloblastosis oncogene
  • Other Designations:
  • gag-myb protein,myeloblastosis proto-oncogene product,tumor-specific myb protein
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