Myb polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using Myb polyclonal antibody (Cat # PAB10189) in COS-7 cell lysates.
Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce Myb modification.
Lane 3 shows detection of bands at 75, 98, and 125 KDa corresponding to overexpressed Myb (arrows) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
Nitrocellulose 0.45 um membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS.
The primary antibody was used at 1:500.
Personal communication, J. Bies, NCI NIH, Bethesda.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Myb.
Immunogen
A synthetic peptide corresponding to internal region of mouse Myb.
Host
Rabbit
Reactivity
Chimpanzee, Gorilla, Horse, Human, Monkey, Mouse, Opossum, Orangutan, Pig, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:25000)
Western Blot (1:250-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using Myb polyclonal antibody (Cat # PAB10189) in COS-7 cell lysates.
Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce Myb modification.
Lane 3 shows detection of bands at 75, 98, and 125 KDa corresponding to overexpressed Myb (arrows) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
Nitrocellulose 0.45 um membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS.
The primary antibody was used at 1:500.
Personal communication, J. Bies, NCI NIH, Bethesda.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Myb
Entrez GeneID
17863Protein Accession#
NP_034978;P06876Gene Name
Myb
Gene Alias
AI550390, M16449, MGC18531, c-myb
Gene Description
myeloblastosis oncogene
Gene Ontology
HyperlinkOther Designations
gag-myb protein|myeloblastosis proto-oncogene product|tumor-specific myb protein
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Publication Reference
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Stress-induced inactivation of the c-Myb transcription factor through conjugation of SUMO-2/3 proteins.
Sramko M, Markus J, Kabat J, Wolff L, Bies J.
The Journal of Biological Chemistry 2006 Dec; 281(52):40065.
Application：IP, WB-Tr, Monkey, COS-7 cells.
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Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.
Bies J, Markus J, Wolff L.
The Journal of Biological Chemistry 2002 Mar; 277(11):8999.
Application：IP, WB-Tr, Monkey, COS-7 cells.
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Stress-induced inactivation of the c-Myb transcription factor through conjugation of SUMO-2/3 proteins.
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