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Last updated: 2017/12/10
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Cd97 polyclonal antibody

  • Catalog # : PAB10186
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Cd97.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-512 of mouse Cd97.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse, Rat
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:1000-1:5000)
    Immunofluorescence (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using Cd97 polyclonal antibody (Cat # PAB10186) shows detection of bands corresponding to free Fc-Cd97- (5EGF) (lower arrowhead) and Fc-Cd97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells.
    The left lane contains lysate from cells transfected with control DNA.
    The right lane contains lysate from COS cells expressing Fc-Cd97- (5EGF).
    No staining was noted from bone marrow lysates taken from Cd97 knockout mice.
    The identity of the band at ~65 KDa appearing in all lanes is not known.
    The formation of the Cd97 complex is currently under investigation.
    Approximately 10 uL of lysate was used in each lane.
    A 1:1,000 dilution of the primary antibody was used.
    The image was processed using a 10-sec exposure.
    Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
  • Immunofluorescence
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Protein Accession#:
  • NP_036055;Q9Z0M6
  • Gene Name:
  • Cd97
  • Gene Alias:
  • AA409984,TM7LN1
  • Gene Description:
  • CD97 antigen
  • Other Designations:
  • EGF-TM7 receptor,OTTMUSP00000025113
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