CASZ1 polyclonal antibody
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More Files
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Western Blot
Western blot using CASZ1 polyclonal antibody (Cat # PAB10183) shows detection of a band ~125 kDa in size corresponding to CASZ1 (arrowhead).
Lane 1 contains a lysate from AS cells transfected with empty vector.
Lane 2 contains lysate from AS cells transfected with CASZ1 (predicted MW 125 kDa).
Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot).
Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA.
Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown).
Personal Communication. Carol Thiele, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CASZ1.
Immunogen
A synthetic peptide corresponding to N-terminus of human CASZ1.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:25000-1:100000)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot using CASZ1 polyclonal antibody (Cat # PAB10183) shows detection of a band ~125 kDa in size corresponding to CASZ1 (arrowhead).
Lane 1 contains a lysate from AS cells transfected with empty vector.
Lane 2 contains lysate from AS cells transfected with CASZ1 (predicted MW 125 kDa).
Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot).
Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA.
Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown).
Personal Communication. Carol Thiele, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CASZ1
Entrez GeneID
54897Protein Accession#
AK092289;CAI22571Gene Name
CASZ1
Gene Alias
CST, FLJ12223, FLJ20321, SRG, ZNF693, dJ734G22.1
Gene Description
castor zinc finger 1
Omim ID
609895Gene Ontology
HyperlinkGene Summary
zinc finger|survival related|survival-related|zinc finger protein 693
Other Designations
OTTHUMP00000002017|castor homolog 1, zinc finger|survival related|survival-related|zinc finger protein 693
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