Cebpd polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Cebpd polyclonal antibody (Cat # PAB10182) shows detection of endogenous protein in whole cell extracts from mouse primary macrophage cells.
The blot shows staining as follows : untreated wild-type (WT) cells (lane 1), WT cells treated 3 h with LPS (lane 2) and Cebpd knock-out cells treated 3H with LPS (lane 3).
The band at ~35 kDa, indicated by the arrowhead, corresponds to endogenous Cebpd.
Primary antibody was used at 1 : 15,000.
ECL was used for detection.
Personal communication, E.Sterneck, NCI, Frederick, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Cebpd.
Immunogen
Recombinant protein corresponding to N-terminus of mouse Cebpd.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
No cross-reactivity is observed with mouse C/EBPalpha or C/EBPbeta.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:9000)
Western Blot (1:1000-1:15000)
Immunoprecipitation (0.8-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Cebpd polyclonal antibody (Cat # PAB10182) shows detection of endogenous protein in whole cell extracts from mouse primary macrophage cells.
The blot shows staining as follows : untreated wild-type (WT) cells (lane 1), WT cells treated 3 h with LPS (lane 2) and Cebpd knock-out cells treated 3H with LPS (lane 3).
The band at ~35 kDa, indicated by the arrowhead, corresponds to endogenous Cebpd.
Primary antibody was used at 1 : 15,000.
ECL was used for detection.
Personal communication, E.Sterneck, NCI, Frederick, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cebpd
Entrez GeneID
12609Protein Accession#
NP_031705;Q00322Gene Name
Cebpd
Gene Alias
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Gene Description
CCAAT/enhancer binding protein (C/EBP), delta
Gene Ontology
HyperlinkGene Summary
O
Other Designations
CCAAT/enhancer binding protein delta
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Publication Reference
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Proteasome-mediated CCAAT/enhancer-binding protein delta (C/EBPdelta) degradation is ubiquitin-independent.
Zhou S, Dewille JW.
The Biochemical Journal 2007 Jul; 405(2):341.
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c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney.
Thangaraju M, Ananth S, Martin PM, Roon P, Smith SB, Sterneck E, Prasad PD, Ganapathy V.
The Journal of Biological Chemistry 2006 Sep; 281(37):26769.
Application：IF, Mouse, Mouse kidney.
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CCAAT/enhancer-binding protein delta activates the Runx2-mediated transcription of mouse osteocalcin II promoter.
Shin CS, Jeon MJ, Yang JY, Her SJ, Kim D, Kim SW, Kim SY.
Journal of Molecular Endocrinology 2006 Jun; 36(3):531.
Application：IP, WB-Ce, Mouse, Mouse 3T3-L1, C3H10T1/2, calvarial cells, MC3T3-E1, ROS, ST2 cells.
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Proteasome-mediated CCAAT/enhancer-binding protein delta (C/EBPdelta) degradation is ubiquitin-independent.
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