Rabbit polyclonal antibody raised against synthetic peptide of PCNA.
A synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA.
This antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:10000-1:50000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using PCNA polyclonal antibody (Cat # PAB10179) shows detection of a predominant bandat ~36 KDa corresponding to PCNA (arrowhead lane 2). Peptide competition effectively eliminates specific band reactivity (Lane 1). For peptide competition, the antibody waspre-incubated with 100 ug (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4°C, followed by abrief centrifugation and then reaction with the membrane. Each lane contains a lysate from 105 cells from the marine dinoflagellate Prorocentrum minimum. Cells were separatedby SDS-PAGE and transferred onto nitrocellulose using standard techniques. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was followed by washes and reaction with HRPGt-a-Rabbit IgG [H&L] MX. Detection was by ECL using a 30 sec exposure time. Personnel Communication. S. Lin,U.Conn.