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Last updated: 2016/12/11
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Eif2ak3 polyclonal antibody

  • Catalog # : PAB10175
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Eif2ak3.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 601-1115 of mouse Eif2ak3.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500-1:3000)
    Immunoprecipitation (10-30ul)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using Eif2ak3 polyclonal antibody (Cat # PAB10175) to detect Eif2ak3 in cell lysates. 300 ug Eif2ak3 over-expressing 293T cell lysate (lanes 1 & 2), or 800 ug wild type (Lanes 3 & 4), and Eif2ak3 knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15 uL anti-Eif2ak3, followed by immunobloting with 1:1000 dilution of Eif2ak3 polyclonal antibody in 5% milk/TBST buffer.
    Lane 1, 293T cells over-expressing Myc-Eif2ak3 wt, Lane 2, 293T cells over-expressing Myc-Eif2ak3 K618A.
    Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Eif2ak3 polyclonal antibody (Cat # PAB10175).
    Positive staining signal observed in wild type mouse sample with Eif2ak3 polyclonal antibody staining only (B) , but not in the knock out mouse sample (C) and pre-immune serum staining (A) .
    The Eif2ak3 polyclonal antibody (Cat # PAB10175) was diluted in 1 : 1,000.
    Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • AAH54809;Q9Z2B5
  • Gene Name:
  • Eif2ak3
  • Gene Alias:
  • AI427929,Pek,Perk
  • Gene Description:
  • eukaryotic translation initiation factor 2 alpha kinase 3
  • Other Designations:
  • -
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