Western blot using TP53I3 polyclonal antibody (Cat # PAB10164) on RKO cells transfected with TP53I3 without insert (lane 1), transfected with pCEP4-TP53I3 (lane 2), under control conditions (lane3) and treated with adriamycin to activate wild-type p53 (lane 4). Also used on MCF-7 cells transfected with pCEP4 without insert (lane 5) and pCEP4-TP53I3 (lane 6).
The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq