Notum polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of HepG2 cell lysate (11 ug/lane) using Notum polyclonal antibody (Cat # PAB10141) (10 ug/mL).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Notum.
Immunogen
Recombinant protein corresponding to full length mouse Notum.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western blot (5 to 10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of HepG2 cell lysate (11 ug/lane) using Notum polyclonal antibody (Cat # PAB10141) (10 ug/mL).Immunohistochemistry
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Gene Info — Notum
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Publication Reference
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HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.
Giráldez AJ, Copley RR, Cohen SM.
Developmental Cell 2002 May; 2(5):667.
Application：IF, IHC, IP, WB-Tr, Insect, S2 cells, Wing discs.
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Characterization of mammalian orthologues of the Drosophila osa gene: cDNA cloning, expression, chromosomal localization, and direct physical interaction with Brahma chromatin-remodeling complex.
Kozmik Z, Machon O, Kralova J, Kreslova J, Paces J, Vlcek C.
Genomics 2001 Apr; 73(2):140.
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LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic.
Riese J, Yu X, Munnerlyn A, Eresh S, Hsu SC, Grosschedl R, Bienz M.
Cell 1997 Mar; 88(6):777.
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HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.
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