PPAP2B polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using PPAP2B polyclonal antibody (Cat # PAB10123) on bacterially expressed PPAP2B protein when untreated (-) and treated with with 0.1 mM IPTG (isopropyl-beta-D-thiogalactopyranoside) (+) at a dilution of 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPAP2B.
Immunogen
A synthetic peptide of human PPAP2B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western blot (10 to 15 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using PPAP2B polyclonal antibody (Cat # PAB10123) on bacterially expressed PPAP2B protein when untreated (-) and treated with with 0.1 mM IPTG (isopropyl-beta-D-thiogalactopyranoside) (+) at a dilution of 10 ug/mL . -
Gene Info — PPAP2B
Entrez GeneID
8613Gene Name
PPAP2B
Gene Alias
Dri42, LPP3, MGC15306, PAP-2b, PAP2-b, PAP2-beta, VCIP
Gene Description
phosphatidic acid phosphatase type 2B
Omim ID
607125Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
OTTHUMP00000009855|lipid phosphate phosphohydrolase 3|phosphatidic acid phosphohydrolase 2b|type-2 phosphatidic acid phosphatase-beta|vascular endothelial growth factor and type I collagen inducible
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Interactome
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Publication Reference
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Ras-MAP kinase signaling by lysophosphatidic acid and other G protein-coupled receptor agonists.
Kranenburg O, Moolenaar WH.
Oncogene 2001 Mar; 20(13):1540.
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Molecular cloning of two alternatively spliced forms of human phosphatidic acid phosphatase cDNAs that are differentially expressed in normal and tumor cells.
Leung DW, Tompkins CK, White T.
DNA and Cell Biology 1998 Apr; 17(4):377.
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Plasma membrane fractions from rat liver contain a phosphatidate phosphohydrolase distinct from that in the endoplasmic reticulum and cytosol.
Jamal Z, Martin A, Gomez-Muñoz A, Brindley DN.
The Journal of Biological Chemistry 1991 Feb; 266(5):2988.
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Ras-MAP kinase signaling by lysophosphatidic acid and other G protein-coupled receptor agonists.
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