Arhgef2 polyclonal antibody
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More Files
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Specification
Product Description
Sheep polyclonal antibody raised against partial recombinant Arhgef2.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 2-447 of Arhgef2.
Host
Sheep
Reactivity
Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western blot (4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using Arhgef2 polyclonal antibody (Cat # PAB10113) on HEK293 cell transfected with vector alone (lane 1), Arhgef2-short (lane 2) and Arhgef2-long (lane 3). -
Gene Info — Arhgef2
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Publication Reference
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The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells.
Glaven JA, Whitehead I, Bagrodia S, Kay R, Cerione RA.
The Journal of Biological Chemistry 1999 Jan; 274(4):2279.
Application:IF, Mouse, NIH/3T3 cells.
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Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein.
Glaven JA, Whitehead IP, Nomanbhoy T, Kay R, Cerione RA.
The Journal of Biological Chemistry 1996 Nov; 271(44):27374.
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Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C.
Whitehead I, Kirk H, Tognon C, Trigo-Gonzalez G, Kay R.
The Journal of Biological Chemistry 1995 Aug; 270(31):18388.
Application:WB-Tr, Mouse, NIH/3T3 cells.
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The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells.
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