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Last updated: 2017/7/23
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Angpt1 polyclonal antibody

  • Catalog # : PAB10092
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Angpt1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of mouse Angpt1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:800)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with Angpt1 polyclonal antibody (Cat # PAB10092) was diluted 1 : 500 to detect Angptl1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoblotting of Angpt1 polyclonal antibody (Cat # PAB10092) was used at a 1:500 dilution to detect mouse Angptl1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells.
    Lane 1 - wt endothelial cells.
    Lane 2 - mouse Angpt1 (clone 1-8) expressing cells.
    Lane 3 - mouse Angpt1 (clone 1-15) expressing cells.
    Lane 4 - mouse Angpt2 (clone 2-9) expressing cells.
    Approximately 20 ug of each lysate was used for 10% SDS-PAGE.
    Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h.
    After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Angpt1
  • Gene Alias:
  • 1110046O21Rik,Ang-1,Ang1
  • Gene Description:
  • angiopoietin 1
  • Other Designations:
  • Angiopoietin-1
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