Immunohistochemical staining with Angpt1 polyclonal antibody (Cat # PAB10092) was diluted 1 : 500 to detect Angptl1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Immunoblotting of Angpt1 polyclonal antibody (Cat # PAB10092) was used at a 1:500 dilution to detect mouse Angptl1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Angpt1 (clone 1-8) expressing cells. Lane 3 - mouse Angpt1 (clone 1-15) expressing cells. Lane 4 - mouse Angpt2 (clone 2-9) expressing cells. Approximately 20 ug of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.