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Ankrd26 polyclonal antibody

  • Catalog # : PAB10090
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ankrd26.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Human, Mouse, Rat
  • Specificity:
  • This affinity-purified antibody is directed against mouse Ankrd26 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000-1:80000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein.
    Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng.
    Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used.
    Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000.
    Sizeestimation was made by comparison to prestained MW markers as indicated.
    Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Protein Accession#:
  • AAH47067;Q69ZS2
  • Gene Name:
  • Ankrd26
  • Gene Alias:
  • 5730521P14Rik,BC008111,mKIAA1074
  • Gene Description:
  • ankyrin repeat domain 26
  • Other Designations:
  • -
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