Asap1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescent microscopy using Asap1 polyclonal antibody (Cat # PAB10088) shows detection of Asap1 present in mouse NIH/3T3 cells transfected with activated Src.
Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Asap1.
Immunogen
A synthetic peptide corresponding to amino acids 777-787 of mouse Asap1.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Specificity
Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunofluorescence
Immunofluorescent microscopy using Asap1 polyclonal antibody (Cat # PAB10088) shows detection of Asap1 present in mouse NIH/3T3 cells transfected with activated Src.
Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — Asap1
Entrez GeneID
13196Protein Accession#
NP_034156(seealsoAAC98349);Q9QWY8Gene Name
Asap1
Gene Alias
AV239055, Ddef1, PAP, mKIAA1249, s19
Gene Description
ArfGAP with SH# domain, ankyrin repeat and PH domain1
Gene Ontology
HyperlinkGene Summary
O
Other Designations
development and differentiation enhancing
-
Publication Reference
-
Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.
Liu Y, Yerushalmi GM, Grigera PR, Parsons JT.
The Journal of Biological Chemistry 2005 Mar; 280(10):8884.
Application：IF, IP, WB-Tr, Human, HEK 293, REF52 cells.
-
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.
Kruljac-Letunic A, Moelleken J, Kallin A, Wieland F, Blaukat A.
The Journal of Biological Chemistry 2003 Aug; 278(32):29560.
Application：WB, Mouse, PC-12 cells, MEFs.
-
The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.
Liu Y, Loijens JC, Martin KH, Karginov AV, Parsons JT.
Molecular Biology of the Cell 2002 Jun; 13(6):2147.
Application：IF, IP, WB-Tr, Human, 10T1/2, REF52 cells.
-
Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com