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Last updated: 2017/2/19
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Asap1 (phospho Y782) polyclonal antibody

  • Catalog # : PAB10087
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Asap1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y782 of mouse Asap1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • Reactivity occurs against mouse ASAP1 pY782 protein and This antibody is specific to the phosphorylated form of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:16000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (20-40 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent microscopy using Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) shows detection of phosphorylated Asap1 present in mouse NIH/3T3 cells transfected with activated Src.
    Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
    Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_034156(seealsoAAC98349);Q9QWY8
  • Gene Name:
  • Asap1
  • Gene Alias:
  • AV239055,Ddef1,PAP,mKIAA1249,s19
  • Gene Description:
  • ArfGAP with SH# domain, ankyrin repeat and PH domain1
  • Other Designations:
  • development and differentiation enhancing
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