Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
Immunofluorescent microscopy using Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) shows detection of phosphorylated Asap1 present in mouse NIH/3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.