Western blot using ING3 polyclonal antibody (Cat # PAB10081) shows detection of a band at ~55 KDa corresponding to ING3 in RKO cells transfected with ING3 (Lane 2). Control RKO cells do not show detection of this specific band (lane1). The identity of the non-specific bands at 33 KDa and 20 KDa has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human coloncancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membranewas probed with the primary antibody diluted to1:1,000. Incubation was at 4°C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG[H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye isa trademark of LI-COR, Inc.
The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq