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Last updated: 2017/12/10
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ING4 polyclonal antibody

  • Catalog # : PAB10080
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ING4.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 164-175 of human ING4.
  • Host:
  • Goat
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:12000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • ING4 polyclonal antibody (Cat # PAB10080) detects ING4 protein by western blot.
    This antibody was used at 1.0 ug/mL to detect ING4 (Lane 2) present in a U-2 OS whole cell lysate over expressing the protein.
    A control lysate (Lane 3) shows no background staining.
    Comparison to MW markers (Lane 1) indicates detection of a single band at ~29 KDa corresponding to ING4.
    A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.
    LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9UNL4(isoform1);NP_938028(isoform2)
  • Gene Name:
  • ING4
  • Gene Alias:
  • MGC12557,my036,p29ING4
  • Gene Description:
  • inhibitor of growth family, member 4
  • Gene Summary:
  • This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq
  • Other Designations:
  • brain my036 protein,candidate tumor suppressor p33 ING1 homolog
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