ING4 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
ING4 polyclonal antibody (Cat # PAB10080) detects ING4 protein by western blot.
This antibody was used at 1.0 ug/mL to detect ING4 (Lane 2) present in a U-2 OS whole cell lysate over expressing the protein.
A control lysate (Lane 3) shows no background staining.
Comparison to MW markers (Lane 1) indicates detection of a single band at ~29 KDa corresponding to ING4.
A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions.
The protein was transferred to nitrocellulose using standard methods.
After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ING4.
Immunogen
A synthetic peptide corresponding to amino acids 164-175 of human ING4.
Host
Goat
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:3000-1:12000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
ING4 polyclonal antibody (Cat # PAB10080) detects ING4 protein by western blot.
This antibody was used at 1.0 ug/mL to detect ING4 (Lane 2) present in a U-2 OS whole cell lysate over expressing the protein.
A control lysate (Lane 3) shows no background staining.
Comparison to MW markers (Lane 1) indicates detection of a single band at ~29 KDa corresponding to ING4.
A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions.
The protein was transferred to nitrocellulose using standard methods.
After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ING4
Entrez GeneID
51147Protein Accession#
Q9UNL4(isoform1);NP_938028(isoform2)Gene Name
ING4
Gene Alias
MGC12557, my036, p29ING4
Gene Description
inhibitor of growth family, member 4
Omim ID
608524Gene Ontology
HyperlinkGene Summary
This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq
Other Designations
brain my036 protein|candidate tumor suppressor p33 ING1 homolog
-
Interactome
-
Publication Reference
-
A screen for genes that suppress loss of contact inhibition: identification of ING4 as a candidate tumor suppressor gene in human cancer.
Kim S, Chin K, Gray JW, Bishop JM.
PNAS 2004 Nov; 101(46):16251.
-
ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.
Zhang X, Xu LS, Wang ZQ, Wang KS, Li N, Cheng ZH, Huang SZ, Wei DZ, Han ZG.
FEBS Letters 2004 Jul; 570(1-3):7.
Application：WB-Tr, Human, HepG2, Saos-2 cells.
-
The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis.
Garkavtsev I, Kozin SV, Chernova O, Xu L, Winkler F, Brown E, Barnett GH, Jain RK.
Nature 2004 Mar; 428(6980):328.
Application：IHC, WB, Human, Human brain tumors, U87 MG cells.
-
A screen for genes that suppress loss of contact inhibition: identification of ING4 as a candidate tumor suppressor gene in human cancer.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com