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Last updated: 2016/12/4
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IRS1 (phospho S307) polyclonal antibody

  • Catalog # : PAB10078
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S307 of human IRS1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Dog, Human, Monkey, Mouse, Pig, Rat
  • Specificity:
  • Reactivity occurs against human IRS1 pS307 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:250-1:1500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using IRS1 (phospho S307) polyclonal antibody (Cat # PAB10078) shows detection of a band at ~180 KDa believed to represent phosphorylated IRS1 (arrowhead).
    Lane 1 shows staining of human 293 cell lysate.
    Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/mL of anisomysin for 30 min.
    The pronounced staining of the band at 180 KDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown).
    The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competedout by pre-incubation with the immunizing peptide.
    Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking with 5% goat serum, 0.5%BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250.
    Reaction occurred overnight at 4°
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3667
  • Protein Accession#:
  • P35568;NP_005535
  • Gene Name:
  • IRS1
  • Gene Alias:
  • HIRS-1
  • Gene Description:
  • insulin receptor substrate 1
  • Other Designations:
  • -
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