ISG15 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against a recombinant ISG15.
Immunogen
Recombinant protein corresponding to human ISG15.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C prior to restoration. For long term storage store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISG15
Entrez GeneID
9636Gene Name
ISG15
Gene Alias
G1P2, IFI15, UCRP
Gene Description
ISG15 ubiquitin-like modifier
Omim ID
147571Gene Ontology
HyperlinkGene Summary
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM
Other Designations
OTTHUMP00000038464|interferon, alpha-inducible protein (clone IFI-15K)|interferon-induced 17-kDa/15-kDa protein|interferon-stimulated protein, 15 kDa|ubiquitin cross-reactive protein
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Interactome
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Disease
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Publication Reference
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UBP43 (USP18) specifically removes ISG15 from conjugated proteins.
Malakhov MP, Malakhova OA, Kim KI, Ritchie KJ, Zhang DE.
The Journal of Biological Chemistry 2002 Mar; 277(12):9976.
Application：WB, Mouse, Mosue lungs.
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SUMO, ubiquitin's mysterious cousin.
Muller S, Hoege C, Pyrowolakis G, Jentsch S.
Nature Reviews. Molecular Cell Biology 2001 Mar; 2(3):202.
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Ubiquitin and its kin: how close are the family ties?
Jentsch S, Pyrowolakis G.
Trends in Cell Biology 2000 Aug; 10(8):335.
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UBP43 (USP18) specifically removes ISG15 from conjugated proteins.
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