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Last updated: 2016/10/23
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MAD2L1 polyclonal antibody

  • Catalog # : PAB10076
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MAD2L1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 3-13 of human MAD2L1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using MAD2L1 polyclonal antibody (Cat # PAB10076) shows detection of a predominant band at ~24 KDa corresponding to MAD2L1 (arrowhead) present in Jurkat (Lane 1) and HeLa (Lane 2) whole cell lysates using the 800 nmchannel (green).
    The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.
    Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide (lanes 4 and 5 respectively) which completely blocks antibody staining.
    Approximately 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1200.
    Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
    Molecular weight markers were used for siz
  • Immunofluorescence
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 4085
  • Protein Accession#:
  • Q13257;NP_002349
  • Gene Name:
  • MAD2L1
  • Gene Alias:
  • Gene Description:
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • Gene Summary:
  • MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq
  • Other Designations:
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1,MAD2-like 1,MAD2-like protein 1,mitotic arrest deficient, yeast, homolog-like 1
  • Gene Pathway
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