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Last updated: 2018/1/14
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MAD2L2 polyclonal antibody

  • Catalog # : PAB10075
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MAD2L2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 3-14 of human MAD2L2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:7500-1:30000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of MAD2L2 polyclonal antibody (Cat # PAB10075) was used at a 1:500 dilution to detect human MAD2L2 by western blot.
    Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.
    This antibody clearly detects a ~20 KDa band corresponding to human MAD2L2 (predicted molecular weight is 24 KDa).
    Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE.
    Primary antibody was reacted with the membrane at room temperature for 1 h.
    After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of MAD2L2 polyclonal antibody (Cat # PAB10075) shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester,, Uppsala, Sweden.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NM_006341(Human);Q9UI95(Human)
  • Gene Name:
  • MAD2L2
  • Gene Alias:
  • MAD2B,REV7
  • Gene Description:
  • MAD2 mitotic arrest deficient-like 2 (yeast)
  • Gene Summary:
  • The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq
  • Other Designations:
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 2,MAD2 homolog,OTTHUMP00000002273,OTTHUMP00000002275,mitotic arrest deficient homolog-like 2
  • Gene Pathway
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