MAD2L2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Immunoblotting of MAD2L2 polyclonal antibody (Cat # PAB10075) was used at a 1:500 dilution to detect human MAD2L2 by western blot.
Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.
This antibody clearly detects a ~20 KDa band corresponding to human MAD2L2 (predicted molecular weight is 24 KDa).
Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of MAD2L2 polyclonal antibody (Cat # PAB10075) shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAD2L2.
Immunogen
A synthetic peptide corresponding to amino acids 3-14 of human MAD2L2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:7500-1:30000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of MAD2L2 polyclonal antibody (Cat # PAB10075) was used at a 1:500 dilution to detect human MAD2L2 by western blot.
Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.
This antibody clearly detects a ~20 KDa band corresponding to human MAD2L2 (predicted molecular weight is 24 KDa).
Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of MAD2L2 polyclonal antibody (Cat # PAB10075) shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAD2L2
Entrez GeneID
10459Protein Accession#
NM_006341(Human);Q9UI95(Human)Gene Name
MAD2L2
Gene Alias
MAD2B, REV7
Gene Description
MAD2 mitotic arrest deficient-like 2 (yeast)
Omim ID
604094Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq
Other Designations
MAD2 (mitotic arrest deficient, yeast, homolog)-like 2|MAD2 homolog|OTTHUMP00000002273|OTTHUMP00000002275|mitotic arrest deficient homolog-like 2
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Interactome
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Pathway
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Disease
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Publication Reference
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MAD2B is an inhibitor of the anaphase-promoting complex.
Chen J, Fang G.
Genes & Development 2001 Jul; 15(14):1765.
Application：Added, Recombinant protein.
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A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2.
Murakumo Y, Roth T, Ishii H, Rasio D, Numata S, Croce CM, Fishel R.
The Journal of Biological Chemistry 2000 Feb; 275(6):4391.
Application：WB-Ce, Human, U-2 OS cells.
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Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta.
Nelson KK, Schlondorff J, Blobel CP.
The Biochemical Journal 1999 Nov; 343 Pt 3:673.
Application：WB-Re, Recombinant protein.
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MAD2B is an inhibitor of the anaphase-promoting complex.
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