NEK2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis is shown using NEK2 polyclonal antibody (Cat # PAB10072) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 KDa correspond-ing to NEK2 protein. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK2.
Immunogen
A synthetic peptide corresponding to amino acids 287-299 of human NEK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:8000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis is shown using NEK2 polyclonal antibody (Cat # PAB10072) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 KDa correspond-ing to NEK2 protein. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEK2
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Interactome
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Disease
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