PLK1 (phospho T210) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various PLK1 mutation constructs.
Blots were reacted with PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069, panel A) and pan reactive anti-PLK1 (panel B).
Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting.
Using a 1:1000 dilution, PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) detects a single band corresponding to endogenousplk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in thesemutants.
Personal communication Hai Jiang, Northwestern Univ.
Western Blot (Cell lysate)
Western blot analysis is shown using PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) to detect endogenousprotein present in a Mouse A-20 whole cell lysate (arrowhead).
Comparison to amolecular weight marker indicates a band of ~68 KDa corresponding to PLK1 protein.
It is suggested to use a nuclear extract fromsynchronized cells to greatly increase theabundance of this protein in preparations.
The blot was incubated with a 1 : 500 dilutionof the antibody at room temperaturefollowed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human breast carcinoma tissue.
Tissue was formalin-fixed and paraffinembedded.
Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human colon carcinoma tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PLK1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T210 of human PLK1.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Clawed frog, Dog, Flies, Human, Mouse, Pig, Rat
Specificity
This antibody is specific to phosphorylated human Plk-1 protein at the pT210 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:3000-1:12000)
Western Blot (1:200-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various PLK1 mutation constructs.
Blots were reacted with PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069, panel A) and pan reactive anti-PLK1 (panel B).
Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting.
Using a 1:1000 dilution, PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) detects a single band corresponding to endogenousplk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in thesemutants.
Personal communication Hai Jiang, Northwestern Univ.Western Blot (Cell lysate)
Western blot analysis is shown using PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) to detect endogenousprotein present in a Mouse A-20 whole cell lysate (arrowhead).
Comparison to amolecular weight marker indicates a band of ~68 KDa corresponding to PLK1 protein.
It is suggested to use a nuclear extract fromsynchronized cells to greatly increase theabundance of this protein in preparations.
The blot was incubated with a 1 : 500 dilutionof the antibody at room temperaturefollowed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human breast carcinoma tissue.
Tissue was formalin-fixed and paraffinembedded.
Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human colon carcinoma tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLK1
Entrez GeneID
5347Protein Accession#
P53350;NP_005021Gene Name
PLK1
Gene Alias
PLK, STPK13
Gene Description
polo-like kinase 1 (Drosophila)
Omim ID
602098Gene Ontology
HyperlinkOther Designations
cell cycle regulated protein kinase|polo (Drosophia)-like kinase|polo like kinase|polo-like kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells.
van Vugt MA, Bras A, Medema RH.
Molecular Cell 2004 Sep; 15(5):799.
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Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades.
Weichert W, Schmidt M, Gekeler V, Denkert C, Stephan C, Jung K, Loening S, Dietel M, Kristiansen G.
The Prostate 2004 Aug; 60(3):240.
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Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.
van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH.
The Journal of Biological Chemistry 2004 Aug; 279(35):36841.
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Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells.
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