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Last updated: 2016/12/4
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PLK1 (phospho T210) polyclonal antibody

  • Catalog # : PAB10069
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PLK1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T210 of human PLK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Clawed frog, Dog, Flies, Human, Mouse, Pig, Rat
  • Specificity:
  • This antibody is specific to phosphorylated human Plk-1 protein at the pT210 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:12000)
    Western Blot (1:200-1:2000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various PLK1 mutation constructs.
    Blots were reacted with PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069, panel A) and pan reactive anti-PLK1 (panel B).
    Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting.
    Using a 1:1000 dilution, PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) detects a single band corresponding to endogenousplk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in thesemutants.
    Personal communication Hai Jiang, Northwestern Univ.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis is shown using PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) to detect endogenousprotein present in a Mouse A-20 whole cell lysate (arrowhead).
    Comparison to amolecular weight marker indicates a band of ~68 KDa corresponding to PLK1 protein.
    It is suggested to use a nuclear extract fromsynchronized cells to greatly increase theabundance of this protein in preparations.
    The blot was incubated with a 1 : 500 dilutionof the antibody at room temperaturefollowed by detection using standard techniques.
    Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human breast carcinoma tissue.
    Tissue was formalin-fixed and paraffinembedded.
    Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human colon carcinoma tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5347
  • Protein Accession#:
  • P53350;NP_005021
  • Gene Name:
  • PLK1
  • Gene Alias:
  • PLK,STPK13
  • Gene Description:
  • polo-like kinase 1 (Drosophila)
  • Other Designations:
  • cell cycle regulated protein kinase,polo (Drosophia)-like kinase,polo like kinase,polo-like kinase
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