SH3BP2 (phospho S427) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis is shown using SH3BP2 (phospho S427) polyclonal antibody (Cat # PAB10066) to detect endogenous protein present in unstimulated human whole cell lysates.
The bandas indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B).
Comparison to a molecular weight marker indicatesa band of ~60 KDa corresponding to human SH3BP2 protein.
The blot was incubated with a 1:500 dilutionof the antibody at room temperature followed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SH3BP2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S427 of human SH3BP2.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to phosphorylated human SH3BP2 protein at the pS427 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis is shown using SH3BP2 (phospho S427) polyclonal antibody (Cat # PAB10066) to detect endogenous protein present in unstimulated human whole cell lysates.
The bandas indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B).
Comparison to a molecular weight marker indicatesa band of ~60 KDa corresponding to human SH3BP2 protein.
The blot was incubated with a 1:500 dilutionof the antibody at room temperature followed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SH3BP2
Entrez GeneID
6452Protein Accession#
P78314;NP_003014;SH3BP2Gene Name
SH3BP2
Gene Alias
3BP2, CRBM, CRPM, FLJ42079, RES4-23
Gene Description
SH3-domain binding protein 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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A missense mutation in the SH3BP2 gene on chromosome 4p16.3 found in a case of nonfamilial cherubism.
Imai Y, Kanno K, Moriya T, Kayano S, Seino H, Matsubara Y, Yamada A.
The Cleft Palate-Craniofacial Journal 2003 Nov; 40(6):632.
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Novel mutation in the gene encoding c-Abl-binding protein SH3BP2 causes cherubism.
Lo B, Faiyaz-Ul-Haque M, Kennedy S, Aviv R, Tsui LC, Teebi AS.
American Journal of Medical Genetics. Part A 2003 Aug; 121A(1):37.
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The chaperone protein 14-3-3 interacts with 3BP2/SH3BP2 and regulates its adapter function.
Foucault I, Liu YC, Bernard A, Deckert M.
The Journal of Biological Chemistry 2003 Feb; 278(9):7146.
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A missense mutation in the SH3BP2 gene on chromosome 4p16.3 found in a case of nonfamilial cherubism.
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