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Last updated: 2017/11/19
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SH3BP2 (phospho S427) polyclonal antibody

  • Catalog # : PAB10066
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of SH3BP2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S427 of human SH3BP2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to phosphorylated human SH3BP2 protein at the pS427 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis is shown using SH3BP2 (phospho S427) polyclonal antibody (Cat # PAB10066) to detect endogenous protein present in unstimulated human whole cell lysates.
    The bandas indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B).
    Comparison to a molecular weight marker indicatesa band of ~60 KDa corresponding to human SH3BP2 protein.
    The blot was incubated with a 1:500 dilutionof the antibody at room temperature followed by detection using standard techniques.
    Personal communication Steven Pelech, Kinexus Inc.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 6452
  • Protein Accession#:
  • P78314;NP_003014;SH3BP2
  • Gene Name:
  • SH3BP2
  • Gene Alias:
  • 3BP2,CRBM,CRPM,FLJ42079,RES4-23
  • Gene Description:
  • SH3-domain binding protein 2
  • Gene Summary:
  • The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • -
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