Western blot analysis is shown using SH3BP2 (phospho S427) polyclonal antibody (Cat # PAB10066) to detect endogenous protein present in unstimulated human whole cell lysates. The bandas indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicatesa band of ~60 KDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilutionof the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.
The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq