SKP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SKP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 152-163 of human SKP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SKP1
Entrez GeneID
6500Protein Accession#
P63208;P63208Gene Name
SKP1
Gene Alias
EMC19, MGC34403, OCP-II, OCP2, SKP1A, TCEB1L, p19A
Gene Description
S-phase kinase-associated protein 1
Omim ID
601434Gene Ontology
HyperlinkGene Summary
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq
Other Designations
RNA polymerase II elongation factor-like protein OCP2|cyclin A/CDK2-associated p19|organ of Corti protein 2|transcription elongation factor B (SIII), polypeptide 1-like
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Interactome
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Pathway
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Disease
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Publication Reference
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NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases.
Liu J, Furukawa M, Matsumoto T, Xiong Y.
Molecular Cell 2002 Dec; 10(6):1511.
Application:WB-Tr, Human, HEK 293T, HeLa cells.
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ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y.
Molecular Cell 1999 Apr; 3(4):535.
Application:WB, Human, HeLa cells.
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Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A.
Michel JJ, Xiong Y.
Cell Growth & Differentiation: the Molecular Biology Journal of the American Association for Cancer 1998 Jun; 9(6):435.
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A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.
Feldman RM, Correll CC, Kaplan KB, Deshaies RJ.
Cell 1997 Oct; 91(2):221.
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SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ.
Cell 1996 Jul; 86(2):263.
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NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases.
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