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Last updated: 2016/12/4
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SMARCAL1 polyclonal antibody

  • Catalog # : PAB10063
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SMARCAL1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 80-96 of human SMARCAL1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000-1:64000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 1.
  • Mutant chromatin remodeling protein SMARCAL1 causes Schimke immuno-osseous dysplasia.
    Boerkoel CF, Takashima H, John J, Yan J, Stankiewicz P, Rosenbarker L, Andre JL, Bogdanovic R, Burguet A, Cockfield S, Cordeiro I, Frund S, Illies F, Joseph M, Kaitila I, Lama G, Loirat C, McLeod DR, Milford DV, Petty EM, Rodrigo F, Saraiva JM, Schmidt B, Smith GC, Spranger J, Stein A, Thiele H, Tizard J, Weksberg R, Lupski JR, Stockton DW.Nat Genet. 2002 Feb;30(2):215-20. Epub 2002 Jan 22.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using SMARCAL1 polyclonal antibody (Cat # PAB10063) shows detection of a band ~106 KDa band (arrowhead) corresponding to SMARCAL1 in human derived cultured cell lysates (HeLa nuclear extract at lane 1, HeLa at lane 2, A-431 at lane 3, Jurkat at lane 4, and 293 at lane 5).
    Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of SMARCAL1 polyclonal antibody.
    Signal was detected using standard techniques.
    SMARCAL1 is the band seen between the 97 and 116kD markers.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9NZC9;NP_003003
  • Gene Name:
  • SMARCAL1
  • Gene Alias:
  • HARP,HHARP
  • Gene Description:
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq
  • Other Designations:
  • HepA-related protein,OTTHUMP00000164057,SMARCA-like protein 1,SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a-like 1
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