SMARCAL1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using SMARCAL1 polyclonal antibody (Cat # PAB10063) shows detection of a band ~106 KDa band (arrowhead) corresponding to SMARCAL1 in human derived cultured cell lysates (HeLa nuclear extract at lane 1, HeLa at lane 2, A-431 at lane 3, Jurkat at lane 4, and 293 at lane 5).
Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of SMARCAL1 polyclonal antibody.
Signal was detected using standard techniques.
SMARCAL1 is the band seen between the 97 and 116kD markers.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMARCAL1.
Immunogen
A synthetic peptide corresponding to amino acids 80-96 of human SMARCAL1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000-1:64000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using SMARCAL1 polyclonal antibody (Cat # PAB10063) shows detection of a band ~106 KDa band (arrowhead) corresponding to SMARCAL1 in human derived cultured cell lysates (HeLa nuclear extract at lane 1, HeLa at lane 2, A-431 at lane 3, Jurkat at lane 4, and 293 at lane 5).
Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of SMARCAL1 polyclonal antibody.
Signal was detected using standard techniques.
SMARCAL1 is the band seen between the 97 and 116kD markers.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMARCAL1
Entrez GeneID
50485Protein Accession#
Q9NZC9;NP_003003Gene Name
SMARCAL1
Gene Alias
HARP, HHARP
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq
Other Designations
HepA-related protein|OTTHUMP00000164057|SMARCA-like protein 1|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a-like 1
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Interactome
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Disease
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Publication Reference
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Mutant chromatin remodeling protein SMARCAL1 causes Schimke immuno-osseous dysplasia.
Boerkoel CF, Takashima H, John J, Yan J, Stankiewicz P, Rosenbarker L, André JL, Bogdanovic R, Burguet A, Cockfield S, Cordeiro I, Fründ S, Illies F, Joseph M, Kaitila I, Lama G, Loirat C, McLeod DR, Milford DV, Petty EM, Rodrigo F, Saraiva JM, Schmidt B, Smith GC, Spranger J, Stein A, Thiele H, Tizard J, Weksberg R, Lupski JR, Stockton DW.
Nature Genetics 2002 Feb; 30(2):215.
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Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse.
Coleman MA, Eisen JA, Mohrenweiser HW.
Genomics 2000 May; 65(3):274.
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Mutant chromatin remodeling protein SMARCAL1 causes Schimke immuno-osseous dysplasia.
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